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General

  • A growth medium or culture medium is a substance in which microorganisms or cells can grow.[1] There are different types of media for growing different types of cells.
  • Types of growth media are: Nutrient media , Minimal media , Selective media , Differential media .
  • There are two major types of growth media: those used for cell culture, which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth.

Technology

  • Cell culture technology is rapidly evolving. At first these developments mainly occurred at a laboratory scale, since the culture media requirements for the propagation of animal cells are very complex and in fact are still largely unknown for many cell types.

Process

  • The quality of the media depends directly upon the quality of the raw materials used for their preparation. Water is the most important raw material used for the preparation of culture media. The parameters to be checked are presence of copper ions, conductivity and pH. Ideally there should be no copper ions present in water because it is inhibitory for the growth of microorganisms.

Market

  • The worldwide cell culture market is estimated at $1.02 billion in 2005 and is expected to grow at a compound annual rate of 12.7 percent to cross $1.86 billion by 2010. With advances in product development, demand for specialty and custom serum-free media, particularly in biomanufacturing applications, will drive market growth.
  • In India, companies like Sigma Aldrich, Invitrogen, HyClone, Alfa Laval, HiMedia Labs, Cambrex, Corning Life Sciences are offering a range of products and services in cell culture space.
  • Several Indian biopharma companies are at the sourcing end for these markets, and these are the ones that require quality assured, consistent and cost-effective raw material supplies with various quality systems and certification program.

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